JN - Fakultas Kedokteran (C)http://hdl.handle.net/123456789/96572024-03-28T12:48:43Z2024-03-28T12:48:43ZAntagonic Effect of Soursop Leaf Aqueous Extract and Doxorubicin Combination in MCF7 and T47D Breast Cancer CellDEWI, Miranti KaniaTRUSDA, Siti Annisa DeviYUNIARTI, Lellyhttp://hdl.handle.net/123456789/304092022-08-04T08:01:12Z2021-12-01T00:00:00ZAntagonic Effect of Soursop Leaf Aqueous Extract and Doxorubicin Combination in MCF7 and T47D Breast Cancer Cell
DEWI, Miranti Kania; TRUSDA, Siti Annisa Devi; YUNIARTI, Lelly
The success of breast cancer therapy is still not optimal and the side effects caused by breast cancer therapy. The use of standard drug combinations with herbs is often used as co-chemotherapy and is believed to increase the drug's effectiveness. However, research on the antagonistic effect of the drug combination is still minimal. This study examines the anticancer effect of soursop leaf aquoxes extract and the combined impact of doxorubicin on MCF7 and T47D breast cancer cells. This research is pure in vitro experimental study of MCF7 and T47D breast cancer culture cells at the Parasitology Laboratory of the Faculty of Medicine, Universitas Gadjah Mada in August 2018. Toxicity tests were carried out using the method of tetrazolium 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium
bromide (MTT) to calculate cell viability. The IC50 value was obtained by analyzing probit regression calculation using SPSS software. The synergism of this compound with doxorubicin was determined based on the value of the Combination Index (CI) using a combination test with series 1/2 IC50, 3/8 IC50, 1/4 IC50, and 1/8 IC50 and the data
was analyzed using Compusyn 1.0 software. In this study, the effect of soursop leaf preparations will be tested on T47D and MCF7 breast cancer cell cultures and assess the impacts of co-chemotherapy of soursop leaf aqueous extract with doxorubicin. This study showed that IC50 soursop leaf aqueous extract in T47D breast cancer culture
was 84 μg/mL and in MCF7 166.5 μg/mL. In contrast, the combined test showed that soursop leaf aqueous extract was antagonistic with doxorubicin in both T47D and MCF7 cancer cell cultures.
Keywords: Antagonic effect, breast cancer, doxorubicin, MCF7, soursop leaf, T47D
Efek Antagonis Kombinasi Extrak Air Daun Sirsak dan Doksorubisin
pada Kultur Sel Kanker MCF7 and T47D
Abstrak
Keberhasilan terapi kanker payudara saat ini masih belum optimal dan terdapat efek samping yang ditimbulkan dari terapi kanker payudara tersebut. Penggunaan kombinasi obat standar dengan herbal sering digunakan sebagai kokemoterapi dan diyakini dapat meningkatkan efektivitas obat, tetapi penelitian mengenai efek antagonis kombinasi
obat masih sangat terbatas. Penelitian ini mengkaji efek antikanker ekstrak air daun sirsak dan kombinasinya dengan doksorubisin pada sel kanker payudara MCF7 dan T47D. Penelitian ini merupakan eksperimental murni secara in vitro pada sel kanker payudara MCF7 dan T47D di Laboratorium Parasitologi Fakultas Kedokteran Universitas Gadjah Mada periode Agustus 2018. Uji toksisitas dilakukan menggunakan metode tetrazolium
3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) untuk menghitung viabilitas sel. Nilai IC50 didapatkan melalui analisis menggunakan perhitungan regresi probit menggunakan perangkat lunak SPSS. Efek sinergis senyawa ini dengan doksorubisin ditentukan berdasar atas nilai Indeks Kombinasi (IK) menggunakan uji
kombinasi dengan seri 1/2 IC50, 3/8 IC50, 1/4 IC50, dan 1/8 IC50 serta data dianalisis menggunakan perangkat lunak Compusyn 1.0. Efek sediaan daun sirsak pada penelitian ini akan diujikan terhadap kultur sel kanker payudara T47D dan MCF7 serta menilai efek ko-kemoterapi ekstrak air daun sirsak dengan doksorubisin. Hasil penelitian ini
menunjukkan bahwa IC50 ekstrak air daun sirsak pada kultur sel kanker T47D adalah 84 μg/mL dan pada kultur sel kanker MCF7 166.5 μg/mL, sedangkan uji kombinasi memperlihatkan bahwa ekstrak air daun sirsak berefek antagonis dengan doksorubisin pada kultur sel kanker T47D dan MCF7.
Kata kunci: Daun sirsak, doksorubisin, efek antagonis, kanker payudara, MCF7, T47D
2021-12-01T00:00:00ZProbability of hypertension in advancing ages of womenYULIANTO, Fajar AwaliaROMADHONA, NurulROSARIANTO, FebyanaRAHMANDA, VihannisBARLIAN, SalmanTANIA, Tresya AnggiGUNAWAN, Romy ReynaldiFUADANA, Sumayya NuriHAQUE, Aulia UlNATAPUTRI, RifaAMALIA, Aulia NurMARESTA, PaulinaNUGROHO, Harishttp://hdl.handle.net/123456789/278242020-10-19T06:01:57Z2020-08-31T00:00:00ZProbability of hypertension in advancing ages of women
YULIANTO, Fajar Awalia; ROMADHONA, Nurul; ROSARIANTO, Febyana; RAHMANDA, Vihannis; BARLIAN, Salman; TANIA, Tresya Anggi; GUNAWAN, Romy Reynaldi; FUADANA, Sumayya Nuri; HAQUE, Aulia Ul; NATAPUTRI, Rifa; AMALIA, Aulia Nur; MARESTA, Paulina; NUGROHO, Haris
Hypertension is a problem in Indonesia, with 34.1% prevalence. The number reflected the number of hypertensive patients in the 2016 clinic report of Pasirjambu Public Health Center as the most prominent non-communicable disease. This research aimed to discover the specific age of onset and risk factors of hypertension in the village where the health center located. A rapid survey collected the data in May 2017, where 210 women (representing their household) were chosen by randomization inside their respective clusters. Risk factors were analyzed by a robust and parsimonious logistic regression model along with probability count on age as the final prediction. The prevalence of hypertension was 59.5% (95%CI: 52.9, 66.2%). Risk factors for hypertension were age (OR=1.06, p=0.00), stress (OR=1.74, p=0.09) and family history (OR=1.99, p=0.03) but the protective factor was consumption frequency of salty food (OR=0.64, p=0.10). In conclusion, a woman would have a 42.9% chance (95%CI: 33.7, 52.1%) for having hypertension at 40 years old of age after adjusted by other risk factors. Despite only two modifiable risk factors that can be intervened with, it would be worth trying to decrease the pace of onset in hypertension and the prevalence.
Hipertensi merupakan sebuah masalah di Indonesia dengan prevalensi sebesar 34,1%, angka tersebut terlihat dalam laporan tahunan Puskesmas Pasirjambu sebagai penyakit tidak menular terbanyak di wilayah kerjanya. Tujuan penelitian ini adalah mengetahui usia munculnya hipertensi dan faktor risikonya. Pengumpulan data dilakukan melalui survei cepat di bulan Mei 2017, melibatkan 210 wanita yang mewakili rumah tangganya dipilih secara random. Faktor risiko dianalisis menggunakan regresi logistik dengan hasil akhir berupa prediksi kemungkinan. Hasil penelitian menunjukkan prevalensi hipertensi sebesar 59,5% (IK95%: 52,9; 66,2%). Usia (OR=1,06; p=0,00), stress (OR=1,74; p=0,09) dan riwayat hipertensi dalam keluarga (OR=1,99; p=0,03) menjadi faktor risiko, sedangkan frekuensi konsumsi makanan asin (OR=0,64; p=0,10) menjadi faktor protektif. Setelah adjusted terhadap variabel lain, kemungkinan untuk hipertensi seorang wanita usia 40 tahun sebesar 42,9% (IK95%: 33,7; 52,1%). Pencegahan untuk menurunkan prevalensi dan laju insidensi dapat dilakukan dalam usia tersebut walaupun hanya ada dua faktor risiko yang dapat dimodifikasi.
2020-08-31T00:00:00ZThe Resistance of Aedes Aegypti to Permethrin 0.25% Insecticide, Malathion 0.8%, and Transfluthrin 25% in the Universitas Islam Bandung Tamansari CampusAstuti, Ratna Dewi IndiIsmawatiSiswanti, Listya Hanumhttp://hdl.handle.net/123456789/278092020-08-19T02:32:17Z2019-01-01T00:00:00ZThe Resistance of Aedes Aegypti to Permethrin 0.25% Insecticide, Malathion 0.8%, and Transfluthrin 25% in the Universitas Islam Bandung Tamansari Campus
Astuti, Ratna Dewi Indi; Ismawati; Siswanti, Listya Hanum
Massive and long term insecticide use causes resistance of mosquitos to insecticides. This research has a goal for assessing the resistance of Aedes aegypti to the insecticides of permethrin 0.25%, malathion 0.8%, and transfluthrin 25% in the Universitas Islam Bandung Tamansari campus. The Aedes aegypti resistance in the Universitas Islam Bandung Tamansari campus Bandung city to insecticides measured with the susceptibility test in September 2015. The susceptibility test to the permethrin 0.25% and malathion 0.8% insecticides implemented by using WHO standard instruments and methods. The susceptibility test to transfluthrin 25% implemented by using commercial insecticide according to the usage suggestion. The total mosquitos that died after the exposure of permethrin 0.25%, transfluthrin 25%, and malathion 0.8% for 60 minutes were 20%, 23%, and 80%. The WHO criteria state that mosquitos were still susceptible to insecticides if the death rate is 98–100%, tolerant if the death rate is 80–97%, and mosquitos are resistant if the death rate is less than 80%. In conclusion, the Aedes aegypti mosquitos in the Universitas Islam Bandung Tamansari campus are already resistant to the insecticides permethrin 0.25% and transfluthrin 25% and tolerant to malathion 0.8%.
2019-01-01T00:00:00ZValidation of patient perception instruments for junior doctor performance: a factor analysisKUSMIATI, MiaBAHARI, RafidahHAMID, Noor Aini AbdulSANIP, SuhailaEMILIA, Ovahttp://hdl.handle.net/123456789/265852020-06-02T05:13:56Z2019-04-01T00:00:00ZValidation of patient perception instruments for junior doctor performance: a factor analysis
KUSMIATI, Mia; BAHARI, Rafidah; HAMID, Noor Aini Abdul; SANIP, Suhaila; EMILIA, Ova
2019-04-01T00:00:00Z