BC - Fakultas Kedokteran (C)
http://hdl.handle.net/123456789/18544
2024-03-28T18:17:13ZFeatures of lymphocyte infiltration in lungs of rats given ethanolic extract of white oyster mushrooms and exposure to cigarette smoke
http://hdl.handle.net/123456789/30637
Features of lymphocyte infiltration in lungs of rats given ethanolic extract of white oyster mushrooms and exposure to cigarette smoke
S.B. Rahimah; S. Fitriyana; I.B. Akbar; N. Soetadipura
ABSTRACT: Cigarette smoke is a huge source of free radicals and high levels of chemicals. Exposure to cigarette smoke can cause inflammation and lung destruction. This study aims to look at the effect of ethanolic extracts of white oyster mushrooms in preventing the development
of lymphocyte infiltration in rats exposed to cigarette smoke. This experimental study used 32 rats divided into 4 groups. Group I (positive control, only given drinking water and standard food), group II (negative control) were given drinking water, standard food, and exposure to cigarette smoke 1 hour/day for 6 weeks. Group III (treatment group) were given cigarette smoke exposure 1 hour/day and ethanol extract of white oyster mushroom at a dose of 250 mg/KgBB of mice/day. Group IV (comparison group) were given cigarette smoke exposure 1 hour/day/group and were given N-acetyl cysteine at a dose of 600 mg/day. Lymphocyte infiltration can be seen from the histopathological picture of the rat lungs-stained by haematoxylin eosin staining. The results showed much of lymphocyte infiltration in rats exposed to cigarette smoke, but there was no significant difference in the proportion of lymphocyte infiltration between all groups (P= 0,14), which was assessed using fisher’s exact test. It can be concluded that ethanolic extracts of white oyster mushrooms can prevent development
of lymphocyte infiltration in rats exposed to cigarette smoke.
2020-01-01T00:00:00ZMicronucleus assay and oral hygiene index in smokers
http://hdl.handle.net/123456789/30636
Micronucleus assay and oral hygiene index in smokers
M.M. Damayanti; Y. Kharisma; I.M. Nur; M. Rachmawati; A.H. Hasan; F.A. Yulianto; S.B. Rahimah; W. Maharani
ABSTRACT: Smoking can lead to oral disease. Many people are unaware of the relationship
between smoking habits and oral hygiene index (OHI), up to potential oral diseases.
Micronuclei (MN) is used as an indicator to look at the cytotoxic effects of smoking and as a biomarker for assessing DNA damage. This study aimed to identify a correlation between the amount of MN and the OHI on smoking habits. Sixty-two subjects with more than 5 years of smoking habits were examined. Oral mucosal cells were collected from both sides of the cheeks and slides were prepared and examined for cells with MN where the presence of MN was assessed under 1000x magnification. The OHI was measured to assess dental debris and calculus. The results showed no significant
correlation between MN and OHI of smoking statistically (P = 0.61). The genotoxic effect of smoking caused chromosomal damage in epithelial cells of the oral mucosa and was reflected in the occurrence of MN and smokers’ poor OHI.
2020-01-01T00:00:00ZAcute toxicity test for the ethanolic extract of the white oyster mushroom
http://hdl.handle.net/123456789/30635
Acute toxicity test for the ethanolic extract of the white oyster mushroom
S.B. Rahimah; Y. Kharisma; M.K. Dewi; J. Hartati; W. Maharani
ABSTRACT: The white oyster mushroom (Pleurotus ostreatus Jacq: Fr Kumm) is a mushroom that contains high nutrition and secondary metabolites that have antioxidant and anti-inflammatory effects. The mushroom is widely used for the prevention of various diseases such as hypertension, hypercholesterolemia, and carcinoma. This study aimed to assess the acute toxicity of the ethanolic extract of the white oyster mushroom. The method used in this study was a new method recommended for the acute toxicity test of natural substances. Doses tested in this study ranged from 10 mg/kg body weight to a maximum dose of 5,000 mg/kg body weight. Observations of toxicity tests were conducted within the first 24 hours at each stage and the parameters observed were changes in behavior and the mortality of experimental animals. The results of the acute toxicity test showed that no experimental animal died during the range of dosing, since the smallest dose reached the maximum dose; researchers observed no other changes in animal behavior during ethanol extract administration. White oyster mushroom ethanol extract is relatively safe and does not show any acute toxicity, because it has a wide dose range.
2020-01-01T00:00:00ZEffects of aqueous extract of unripe papaya (Carica papaya L.) on mice milk production
http://hdl.handle.net/123456789/30634
Effects of aqueous extract of unripe papaya (Carica papaya L.) on mice milk production
Y. Kharisma; S.B. Rahimah; H.S. Sastramihardja
ABSTRACT: Breast milk is the best food to meet the nutritional needs of infants. According to the Indonesia Health Survey in 2005, 4–12% of babies in urban areas were exclusively breastfed and 4–5% of babies in rural areas were exclusively breastfed. The objective of this study was to examine the effect of aqueous extract of unripe papaya on milk production by measuring the weight gain and growth of baby mice. An experimental study was conducted with 21 lactating mice divided randomly into three treatment groups with 10 babies each. The first group served as a negative control; it was started at lactation days 4–16. The result was analyzed using ANOVA and followed by a Tukey test with a 95% level of confidence. The baby mice’s weight gains in groups I, II, and III were 1.25 ± 0.62 g/6 hours, 2.25 ± 0.29 g/6 hours, and 2.21 ± 0.28 g/6 hours, respectively. The research showed that aqueous extract of unripe papaya has a better effect than the negative control and has an equivalent effect in improving weight gain and growth of baby mice given luteotropin.
2020-01-01T00:00:00Z