JI - Fakultas Kedokteran (C)

Mangifera indica Linn. Waste Peel Ethanol Extract on Inducing Citrus amblycarpa Hassk. Ochese Antioxidant Activity

2025-05-05T04:49:35Z

Mangifera indica Linn. Waste Peel Ethanol Extract on Inducing Citrus amblycarpa Hassk. Ochese Antioxidant Activity Indriyanti, Raden Anita; Kharisma, Yuktiana; Damayanti, Meta Maulida Background: Excessive production of reactive oxygen species plays a significant role in the proliferation and evolution of numerous high-risk diseases in humans, the antioxidant capacity of bioactive substances is of immense importance. Mangifera indica L., a medicinal plant has high bioactive metabolite contents and contributes to several positive biological processes in the plant. Citrus amblycarpa H.O. contains an excellent source of chemical compounds and the presence of these compounds can be a potential pharmacological activity as antimicrobial, anti-inflammatory, antioxidant, and others. The aim of the study is to assess the effect of Mangifera indica and Citrus amblycarpa peel ethanol extract as an antioxidant agents. Method: Phytochemical screening of M. indica and C. amblycarpa peeled ethanol extract was done to identify alkaloids, polyphenols, flavonoids, anthraquinone, tannins, and terpenoid contents. The antioxidant activity of the extract was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Result: Mangifera indica and Citrus amblycarpa peel ethanol extract have an antioxidant activity of IC50 29,28 μg/mL and IC50 669,52 μg/mL separately, while combination antioxidant activity was 48,05 μg/ mL. Conclusion: Mangifera indica Linn. peel ethanol extract could induce Citrus amblycarpa antioxidant activity producing a potential antioxidant agent.

Pre-Clinical Study: Immunohistochemical Evaluation of Matrix Metalloproteinase-13 on Rabbit Oryctolagus Cuniculus) Socket Healing After Application of Platelet-rich Fibrin with and without Hydroxyapatite

2025-05-05T04:40:30Z

Pre-Clinical Study: Immunohistochemical Evaluation of Matrix Metalloproteinase-13 on Rabbit Oryctolagus Cuniculus) Socket Healing After Application of Platelet-rich Fibrin with and without Hydroxyapatite Damayanti, Meta Maulida; Rachmawati, Meike Background: Tissue engineering technology has been used globally and proven to accelerate wound healing. This study aimed to analyse the effect of adding hydroxyapatite (HA) as a scaffold to platelet-rich fibrin (PRF) as a growth factor in accelerating the wound healing process as seen from the expression of matrix metalloproteinase-13 (MMP-13). Methods: This research is an animal experiment conducted on 18 rabbits (Oryctolagus cuniculus). Rabbits were randomly divided into the following three groups of treatment: (G1) the application of PRF group, (G2) the application of PRF+HA group and (C) the control group without any application. Furthermore, each treatment group was split randomly into three groups of observation time. Periodontal tissue biopsy was performed to analyse the histopathological features that were examined on the basis of the level of MMP-13 immunoexpression. Results: MMP-13 immunoexpression in the PRF+HA group showed better histoscore results, indicating a substantial reduction in MMP-13 values compared with other groups. The healing process was shown to increase with increasing observation time (p<0.05), and the PRF+HA group outperformed the PRF and control groups. On day 3, MMP-13 exhibited a dark brown colour of Immunohistochemistry (IHC), which indicated an increase in the expression value of MMP-13 in the early stages of healing, namely, inflammation. On day 14, light brown IHC was seen, especially in group 2, as a reference that the remodeling process had begun. Conclusions: This study indicates that the administration of PRF and HA was capable of reducing the MMP-13 expression that significantly accelerates the socket healing process. Hydroxyapatite is an alloplastic material that has inherent bioactive properties that support osteoconduction, can bind MMPs, and showed faster healing results based on the observation time as documented by immunohistochemistry.

STRUCTURE-BASED DESIGN THROUGH MOLECULAR DYNAMICS APPROACHES OF THE SMALL-MOLECULE BIOACTIVE COMPOUNDS IN CINNAMON AS INTERLEUKIN- 6 (IL-6) INHIBITORS

2025-05-02T04:10:57Z

STRUCTURE-BASED DESIGN THROUGH MOLECULAR DYNAMICS APPROACHES OF THE SMALL-MOLECULE BIOACTIVE COMPOUNDS IN CINNAMON AS INTERLEUKIN- 6 (IL-6) INHIBITORS Damayanti, Meta Maulida; Rachmawati, M.; Widiyastuti, E.; Kharisma, Y. The lip mucosa's wound healing process necessitates using a material that can reduce inflammation, prevent infection, expedite wound closure, and inhibit scar formation. Interleukin-6 (IL-6) is a crucial modulator of the wound-healing process. Cinnamon is a natural resource with various chemical compounds and pharmacological activities, including wound healing mediated by proinflammatory cytokines such as IL-6. This research aims to identify the lead compounds for IL-6 from Cinnamon based on binding affinity, mode interaction, and stability utilizing molecular docking and dynamics. After categorizing variables such as binding energy and molecular interactions, including hydrogen bonding and hydrophobic interactions, the docking process revealed the potential of the Epicatechin, Catechin, Coumarinic acid, 1,4-benzene dicarboxylic acid, 3,4-dihydroxybenzaldehyde, and Coumarin to interact with IL-6 binding pocket. Furthermore, the stability of these compounds was assessed during a 200 ns simulation, offering satisfactory results with similar profiles based on RMSD, RMSF, SASA, Rg, and intramolecular and intermolecular hydrogen bond parameters. Catechin, Coumarin, and Epicatechin are the most potential candidates based on their affinities calculated by the MM-PBSA approach of -56.847 kJ/mol, -42.573 kJ/mol, and -38.246 kJ/mol, respectively. These are preliminary findings that may be appropriate for additional experimental investigation

The Role of Healing Effect of Lip Balm Application Cinnamomum burmannii on lip wound based on Immunohistochemical Interleukin 6 (IL-6) Levels

2025-05-02T01:25:53Z

The Role of Healing Effect of Lip Balm Application Cinnamomum burmannii on lip wound based on Immunohistochemical Interleukin 6 (IL-6) Levels Damayanti, Meta Maulida; Widiyastuti, Ermina; Rachmawati, Meike The oral mucosa goes through a complicated series of biological healing processes to regain equilibrium after being injured. To heal wounds, we need a material that can heal wounds quickly and well. Cinnamon is widely used for therapy because it has good anti-bacterial, anti-inflammatory, and antioxidant effects. The purpose of this study was to analyze the healing effect of the application of lip balm Cinnamomum on lip incisions based on the level of immunohistochemical interleukin-6. This experimental study was conducted at the laboratory biomedical Faculty of Medicine, Universitas Islam Bandung. A 10 mm length and 4 mm depth incision lip mucous was surgically created in twenty-seven male rats with healthy, 200-300mg of body weight, male, 3-5 months. Animals were divided by simple random sampling were divided into three groups, group 1: the animal treated lip healing with distilled water (control). Group 2: the animal treat lip healing with placebo lip balm, and lastly, group 3: the animal treat lip healing with lip balm Cinnamomum burmannii. Each group performs observation on day-3, 7, and 14. To evaluate the effectiveness of treatment, a lip biopsy performs during each observation time. The immunoexpression IL-6 analysis was assessed by scoring distribution and intensity expression with a Leica microscope. There were significant differences in the level of immunoexpression Interleukin-6 in a group with treatment by lip balm Cinnamomum burmannii. By the time of observation, day 14 of group 2 have a greater level of IL-6 compared with the other groups. Application lip balm Cinnamomum burmanni is effective in faster and good remodeling lip healing process by seen increasing level of IL-6.